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Öğe Antimicrobial evaluation of Tripleurospermum callosum (Boiss. & Heldr.) E. Hossain extracts using in vitro and in vivo Caenorhabditis elegans model against urinary system pathogens(Taylor & Francis Ltd, 2024) Goger, Gamze; Yuksel, Deniz; Goger, Fatih; Kose, Yavuz Bulent; Demirci, FatihTripleurospermum callosum (Boiss. & Heldr.) E. Hossain was recorded in Turkish ethnobotanical data for its use against urinary and respiratory system ailments. Infusion, decoction and 96% ethanol extracts of T. callosum aerial parts were prepared for in vitro antimicrobial activity against urinary system pathogens Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Klebsiella aerogenes ATCC 1348 and Candida albicans ATCC 10231. The non-toxic concentrations of extracts and in vivo antimicrobial assay were performed using C. elegans. The extracts were analysed by Liquid Chromatography Mass Spectrometry (LC-MS/MS) for phytochemical composition. The water extracts were non-toxic at between 5000 and 312 mu g/mL, while 96% ethanol extract at 312 mu g/mL for C. elegans. The infusion extract showed in vivo anti-infective effect 5000-312 mu g/mL against Gram-negative strains. The results indicate a potential role of plant extracts with relatively non-toxic and anti-infective effects against urinary system pathogens.Öğe Assessment of Cota altissima (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities(Walter De Gruyter Gmbh, 2021) Goger, Gamze; Allak, Muhammed; Sen, Ali; Goger, Fatih; Tekin, Mehmet; Ozek, GulmiraPhytochemical profiles of essential oil (EO), fatty acids, and n-hexane (CAH), diethyl ether (CAD), ethyl acetate (CAE) and methanol extracts (CAM) of Cota altissima L. J. Gay (syn. Anthemis altissima L.) were investigated as well as their antioxidant, anti-inflammatory, antidiabetic and antimicrobial activites. The essential oil was characterized by the content of acetophenone (35.8%) and beta-caryophyllene (10.3%) by GC-MS/FID. Linoleic and oleic acid were found as main fatty acids. The major constituents of the extracts were found to be 5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, isorhamnetin glucoside, quercetin and quercetin glucoside by LC-MS/MS. Antioxidant activities of the extracts were determined by scavenging of DPPH and ABTS free radicals. Also, the inhibitory effects on lipoxygenase and alpha-glucosidase enzymes were determined. Antimicrobial activity was evaluated against Gram positive, Gram negative bacteria and yeast pathogens. CAM showed the highest antioxidant activity against DPPH and ABTS radicals with IC50 values of 126.60 and 144.40 mu g/mL, respectively. In the anti-inflammatory activity, CAE demonstrated the highest antilipoxygenase activity with an IC50 value of 105.40 mu g/mL, whereas, CAD showed the best inhibition of a-glucosidase with an IC50 value of 396.40 mu g/mL in the antidiabetic activity. CAH was effective against Staphylococcus aureus at MIC = 312.5 mu g/mL. This is the first report on antidiabetic, anti-inflammatory and antimicrobial activities of different extracts of C. altissima.Öğe Composition and potential of Tanacetum haussknechtii Bornm. Grierson as antioxidant and inhibitor of acetylcholinesterase, tyrosinase, and -amylase enzymes(Taylor & Francis Inc, 2018) Yur, Suleyman; Tekin, Mehmet; Goger, Fatih; Baser, Kemal H. C.; Ozek, Temel; Ozek, GulmiraThe phytochemical composition of essential oils and extracts of Tanacetum haussknechtii were investigated with GC-FID-MS and LC-MS/MS techniques and evaluated against oxidation, acetylcholinesterase, tyrosinase, and -amylase enzymes. The major volatile constituents of T. haussknectii were found to be - and -pinene, and borneol. Caffeoylquinic acid derivatives and flavonoids were detected in the aqueous, alcohol, and ethyl acetate extracts. In DPPH assay, the methanol extracts exhibited the highest activity. TEAC assay resulted with superiority of all methanol and the capitula ethyl acetate extract. In -carotene bleaching assay, linoleic acid was the best protected by the ethyl acetate extract of flower. The flower oil inhibited higher acetylcholinesterase activity than the remaining extracts. The flower ethyl acetate extract was found as the most effective inhibitor of -amylase. The herb and the leaf+stem water extracts possess highest inhibitory effect on tyrosinase.Öğe Myrtle improves renovascular hypertension-induced oxidative damage in heart, kidney, and aortic tissue(Springer, 2022) Arslan, Sule; Ozcan, Ozan; Gurel-Gokmen, Begum; Cevikelli-Yakut, Zatiye Ayca; Saygi, Halil Ibrahim; Sen, Ali; Goger, FatihRenovascular hypertension is defined as the reduction in renal perfusion resulting in sustained hypertension. This study aims to investigate the possible effects of myrtle leaf extract (Myrtus communis L.) on, heart, kidney and aorta tissues in the experimental renovascular hypertension (RVH). 32 male Wistar Albino rats were divided into four groups as control, hypertension, hypertension+ramipril, and hypertension+myrtle leaf extract treatment groups. RVH model was induced by Goldblatt's 2-kidney 1-clip method. 12 weeks later than the treatment blood pressures were recorded and oxidant and antioxidant parameters, tissue factor activity, and histological analysis were determined in the kidney, heart, and aortic tissues. The blood pressure levels of the hypertension group significantly increased compared to the control group. Administration of myrtle leaf extract and ramipril significantly decreased the increased blood pressure. In the hypertension group, oxidative damage increased in the kidney, heart, and aorta tissues. In the histological evaluation of tissues in RVH, heart muscle fibres degenerated. Bowman capsule and glomeruli dilated and tubules damaged in the kidney. Myrtle leaf extract administration regenerated the damages and degenerations. The administration of myrtle leaf extract restored the impaired oxidant-antioxidant balance in the heart, kidney and aorta tissues of hypertensive rats. Myrtle leaf extract can be considered as an alternative antihypertensive treatment target in the prevention of oxidative stress-induced damage in renovascular hypertension.Öğe Phytochemical characterisation of Phlomis linearis Boiss. & Bal and screening for anticholinesterase, antiamylase, antimicrobial, and cytotoxic properties(Tubitak Scientific & Technological Research Council Turkey, 2021) Goger, Gamze; Turkyolu, Ummuhan; Gursen, Ezgi Nur; Yur, Suleyman; Karaduman, Abdullah Burak; Goger, Fatih; Tekin, MehmetIn the present work, essential oil and fatty acids and extracts obtained from aerial parts of Phlomis linearis Boiss. & Bal. were investigated for chemical composition and biological activities. The phytochemical analyses were conducted with gas chromatography-mass spectrometry/flame ionisation detector (GC-MS/FID) and liquid chromatography-mass spectromtetry (LC-MS/MS) techniques. The extracts and essential oil were studied for alpha-amylase and acetylcholinesterase activities with two different spectrophotometric methods. Antimicrobial activities of the extracts were investigated by microdilution. The extracts were evaluated in vitro for cytotoxic effects against cancer and normal cell lines by MTT assay. The essential oil (EO) contained alpha-pinene (12.5%) and beta-caryophyllene (10.7%) as main compounds. Palmitic (26.5%) and nonadecanoic acids (26.6%) were determined as fatty acids. Phytochemical analysis of the extracts found phenolic acids, phlinosides, verbascoside, and flavonoids. The extracts and essential oil demonstrated poor alpha-amylase inhibitory activity. The best acetylcholinesterase inhibitory activity was obtained for diethly ether extract of P. linearis (67.2 +/- 3.4%) at 10 mg /mL concentration. Ethyl acetate extract found to be effective against Staphlococcus aureus at a minimum inhibitory concentration (MIC) of 156.26 mu g/mL. Diethyl ether extract of P. linearis was active on A549 cell lines with an IC50 = 316 +/- 4.16 mu g/mL when compared with cisplatin IC50 = 24.43 +/- 0.14 mu g/mL. To the best of our knowledge, the present work is the first comprehensive report on anti-acetylcholinesterase, anti-alpha-amylase, and antimicrobial activities, as well as cytotoxic effects of P. linearis.Öğe Phytochemical Characterization of Phenolic Compounds by LC-MS/MS and Biological Activities of Ajuga reptans L., Ajuga salicifolia (L.) Schreber and Ajuga genevensis L. from Turkey(Galenos Publ House, 2021) Goger, Gamze; Kose, Yavuz Bulent; Demirci, Fatih; Goger, FatihObjectives: In this study, it was aimed to characterize the phenolic contents of Ajuga reptans L., Ajuga salicifolia (L.) Schreber and Ajuga genevensis L. and to investigate their in vitro antioxidant and antimicrobial activities. Materials and Methods: Air dried aerial parts of A. reptans L., A. salicifolia (L.) Schreber, and A. genevensis L. collected from Turkey were extracted with methanol (70%), and the phenolic composition of the crude extracts was analyzed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) method. To determine the total phenolic content the Folin-Ciocalteu method was used. The radical scavenging activities of the extracts were evaluated by the photometric 1,1-diphenyl-2-picrylhydrazyl radical, and trolox equivalent antioxidant capacity assays (TEAC). Furthermore, Ajuga sp. extracts were tested against Escherichia coli NRRL B3008, Staphylococcus areus ATCC 6538, Salmonella thyphimurium ATCC 13311, Bacillus cereus NRRL B-3711, Candida albicans ATCC 90028, Candida tropicalis ATCC 1369, and Candida parapsilosis ATCC 22019 using the in vitro broth dilution assay. Results: The LC-MS/MS analyses identified 19 compounds. The amount of total phenolics ranged from 30.0 to 42.2 mg gallic acid equivalent/g in all extracts. According to the results of TEAC assay, the tested extracts were found to have relatively high activity at 1.2-1.5 mM concentrations. Ajuga sp. extracts inhibited all tested microorganisms; however, C. albicans, C. tropicalis, and C. parapsilosis exhibited relatively more susceptibility (minimum inhibitory concentration: 156.25 mu g/mL) compared to the bacteria tested. Conclusion: The antioxidant activities of all extracts were determined for the first time by the TEAK method, and the in vitro antimicrobial activity of A. salicifolia was investigated for the first time against selected strains.Öğe Synthesis, antimicrobial and antioxidant activities of pyridyl substituted thiazolyl triazole derivatives(Univ Sao Paulo, Conjunto Quimicas, 2022) Tay, Naime Funda; Duran, Murat; Kayagil, Ismail; Yurttas, Leyla; Goger, Gamze; Goger, Fatih; Demirci, FatihIn this present study, 63 different 5-[4-methyl-2-(pyridin-3/4-yl)thiazole-5-yl]-4-substituted-3-substituted benzylthio-4H-1,2,4-triazole derivatives were synthesized, and evaluated for their in vitro antimicrobial activity against various human pathogenic microorganisms and antioxidant activity. The derivatives were synthesized in a multi-step synthesis procedure including triazole and thiazole ring closure reactions, respectively. The synthesized derivatives (A1-24; B1-39) were screened for their antibacterial, antifungal, and antioxidant activities compared to standard agents. The derivatives possessing 3-pyridyl moiety particularly exhibited relatively high antibacterial activity (MIC= < 3.09-500 mu g/mL) against Gram-positive bacteria, and compounds possessing 4-pyridyl moiety showed remarkable antioxidant activity.
