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Öğe Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury(Mashhad Univ Med Sciences, 2017) Ugurel, Vedat; Cicek, Ahmet Cagatay; Cemek, Mustafa; Demirtas, Selim; Kocaman, A. Tuba; Karaca, TuranObjective(s): To evaluate the protective effect of erdosteine, an antiapoptotic and antioxidant agent, on torsion-detorsion evoked histopathological changes in experimental ovarian ischemia-reperfusion (IR) injury. Materials and Methods: Eighteen female Wistar albino rats were used in control, IR, and IR+Edosteine (IR-E) groups, (n=6 in each). The IR-E group received the erdosteine for seven days before the induction of torsion/retorsion, (10 mg/kg/days). The IR and IR-E groups were exposed to right unilateral adnexal torsion for 3 hr. Three hours later, re-laparotomy was performed, and the right ovaries were surgically excised. Oxidant and antioxidants levels were determined in serum. The ovarian tissue samples were received and fixed with 10% neutral buffered formalin. The sections were stained with H&E, anti-PCNA, and TUNEL. Results: The IR group were showed severe acute inflammation, polynuclear leukocytes and macrophages, stromal oedema and haemorrhage. Treatment with erdosteine in rats significantly retained degenerative changes in the ovary PCNA (+) cell numbers were significantly decreased in the IR and IR-E groups unlike the control group. However, its numbers were significantly increased in the IR-E group unlike the IR group. TUNEL (+) cell numbers were significantly increased in the IR group unlike the control and the IR-E groups. In erdosteine treated group, TUNEL (+) cells were detected significantly less than the IR group (P<0.05). Conclusion: In conclusion, erdosteine maybe a protective agent for ovarian damage and decreasing lipid peroxidation products and leukocytes aggregation after adnexal torsion in animals.Öğe Desferrioxamine effectively attenuates testicular tissue at the end of 3 h of ischemia but not in an equal period of reperfusion(Elsevier Sci Ltd, 2014) Ayvaz, Suleyman; Inan, Mustafa; Aksu, Burhan; Karaca, Turan; Cemek, Mustafa; Ayaz, Ahmet; Basaran, Umit NusretObjective: To investigate the effect of desferrioxamine (DFX) on ipsilateral and contralateral testis damage caused by experimental testis torsion and detorsion. Materials and methods: Forty rats were divided into five groups (n = 8): control, torsion (T), torsion + desferrioxamine (T + DFX), torsion/detorsion (T/D), and torsion/detorsion + desferrioxamine (T/D + DFX). The right testes of the rats were subjected to torsion and detorsion for 3 h each. Thirty minutes before the application of torsion and detorsion, DFX (100 mg/kg) was administered intramuscularly. Blood samples and testicular tissues were examined using specific biochemical and histopathological methods. Results: Ipsilateral and contralateral testis tissue glutathione levels in the T group decreased compared with the control and T + DFX groups. Plasma glutathione peroxidase activity in the T, T/D, and T/D + DFX groups was lower than in the control group. Plasma catalase activity in the T and T/D groups decreased compared with the control group. Ipsilateral mean seminiferous tubule diameter of the T group was lower than that of the T + DFX group. The ipsilateral mean testis biopsy scores in the T and T/D groups were lower than in the control group. Conclusion: The administration of DFX prior to torsion may be useful only for preventing ischemic damage in ipsilateral and contralateral testes. (C) 2013 Journal of Pediatric Urology Company. Published by Elsevier Ltd. All rights reserved.Öğe Desferrioxamine Reduces Oxidative Stress in the Lung Contusion(Hindawi Ltd, 2013) Basaran, Umit Nusret; Ayvaz, Suleyman; Aksu, Burhan; Karaca, Turan; Cemek, Mustafa; Karaboga, Ihsan; Inan, MustafaOur hypothesis in this study is that desferrioxamine (DFX) has therapeutic effects on experimental lung contusions in rats. The rats were divided into four groups (n = 8): control, control+DFX, contusion, and contusion+DFX. In the control+DFX and contusion+DFX groups, 100 mg/kg DFX was given intraperitoneally once a day just after the contusion and the day after the contusion. Contusions led to a meaningful rise in the malondialdehyde (MDA) level in lung tissue. MDA levels in the contusion+DFX group experienced a significant decline. Glutathione levels were significantly lower in the contusion group than in the control group and significantly higher in the contusion+DFX group. Glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels in the contusion group were significantly lower than those in the control group. In the contusion+DFX group, SOD and GPx levels were significantly higher than those in the contusion group. In light microscopic evaluation, the contusion and contusion+DFX groups showed edema, hemorrhage, alveolar destruction, and leukocyte infiltration. However, histological scoring of the contusion+DFX group was significantly more positive than that of the contusion group. The iNOS staining in the contusion group was significantly more intensive than that in all other groups. DFX reduced iNOS staining significantly in comparison to the contusion group. This study showed that DFX reduced oxidative stress in lung contusions in rats and histopathologically ensured the recovery of the lung tissue.Öğe Effects of green tea extract and lactobacillus casei strain shirota on levels of serum minerals, cholesterol triglycerides, glucose and lactate in rats fed on high-carbohydrate and high-lipid diets(2013) Karaca, Turan; Karaboğa, İhsan; Bayıroğlu, Fahri; Comba, Bahat; Cemek, Mustafa; Ayaz, AhmetAMAÇ: Bu çalı?manın amacı, ye?il çay ekstraktı ve Lactobacillus casei strain Shirotanın yüksek karbonhidrat ve lipit içerikli diyetle beslenen ratlarda serum mineralleri (Ca, Cu, Zn, Fe, Mg and Mn), kolesterol, trigliserid, glikoz ve laktat düzeyleri üzerine etkilerinin ara?tırılmasıdır. YÖNTEM: Otuzbe? sağlıklı Wistar albino rat her grupta 5 hayvan ola- cak ?ekilde 7 gruba ayrıldı: Kontrol grubu (A), yüksek-karbonhidratlı diyet grubu (B), yüksek karbonhidratlı diyet ve 8 hafta süreyle probiyotik bakteri ilaveli grup- Lactobacillus casei strain Shirota (C), yüksek karbonhidratlı diyet ve 8 hafta süreyle ye?il çay ekstraktı verilen grup D), yüksek lipit içerikli diyet grubu (E), yüksek lipit içerikli diyet vehafta süreyle probiyotik bakteri ilaveli grup- Lactobacillus casei strain Shirota (F) ve yüksek lipit içerikli diyet ve 8 hafta süreyle ye?il çay ekstraktı ilaveli grup (G). BULGULAR: Serum kolesterol seviyesinin yüksek lipit ve yüksek ipit + ye?il çay ekstraktı ilaveli gruplarda (grup E ve G) kontrole ve diğer deneme gruplarına göre anlamlı olarak artığı tespit edildi p<0.05). Serum trigliserid seviyesi yüksek karbonhidrat + probiyo- ik ilaveli grupta (grup C) diğer deneme gruplarından anlamlı olarak dü?üktü (p<0.05). Yüksek kalorili diyet, ye?il çay ekstraktı ve probiyotik ilavesinin gruplarda serum laktat seviyesi üzerine herhangi bir etkisinin olmadığı görüldü. Serum Ca düzeyinin yanlızca yüksek karbonhidrat içerikli diyet grubunda anlamlı olarak azaldığı tespit edildi (p<0.05). Bununla birlikte, serum Zn ve Fe konsantrasyonlarının yüksek lipit ve yüksek lipit + probiyotik ilaveli gruplarda sırasıyla p<0.05 ve P<0.01 düzeylerinde anlamlı olarak artığı tespit edildi. Serum Mg seviyesinin tüm deneme gruplarında kontrole göreatistiksel olarak anlamlı derecede azaldığı tespit edildi (P<0.01).casei strain Shirota ve ye?il çay ekstraktı alınması yüksek karbon- hidrat diyetli gruplarda serum glikoz seviyesini yüksek lipid diyetli gruba göre anlamlı olarak azalttığı belirlendi (p<0.05). SONUÇ: Ye?il çay ekstraktı ve L. casei strain Shirota kullanımının yüksek enerjili diyetle beslenen ratlarda kan glukoz ve trigliserid seviyelerini dü?ürücü etkisi vardır.Öğe Effects of sphingosylphosphorylcholine against oxidative stress and acute lung injury induced by pulmonary contusion in rats(W B Saunders Co-Elsevier Inc, 2015) Aksu, Burhan; Ayvaz, Suleyman; Aksu, Feyza; Karaca, Turan; Cemek, Mustafa; Ayaz, Ahmet; Demirtas, SelimBackground/purpose: The goal of this study was to evaluate effects of exogenous sphingosylphosphorylcholine (SPC) administration on acute lung injury induced by pulmonary contusion in rats. Methods: Eight animals were included in each of the following five groups: control, contusion, contusion phosphate-buffered solution (PBS), contusion SPC 2, contusion SPC 10. SPC was administered 3 days at a daily two different doses of 2 mu m/ml and 10 mu m/ml intraperitoneally. The severity of lung injury was determined by the neutrophil activation and histological and immunohistochemical changes in the lung. Malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione (GSH) were determined to evaluate the oxidative status in the lung tissue. Results: Treatment with 2 mu M SPC inhibited the increase in lung MDA and NO levels significantly and also attenuated the depletion of SOD, GPx, and GSH in the lung injury induced by pulmonary contusion. These data were supported by histopathological findings. The inducible nitric oxide synthase (iNOS) positive cells and apoptotic cells in the lung tissue were observed to be reduced with the 2 mu M SPC treatment. But, the 10 mu M SPC treatment did not provide similar effects. Conclusions: In conclusion, these findings suggested that 2 mu M SPC can attenuate lung damage in pulmonary contusion by prevention of oxidative stress, inflammatory process and apoptosis. All these findings suggest that low dose SPC may be a promising new therapeutic agent for acute lung injury. (C) 2015 Published by Elsevier Inc.Öğe In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesions(Taylor & Francis Ltd, 2018) Ortac, Deniz; Cemek, Mustafa; Karaca, Turan; Buyukokuroglu, Mehmet E.; Ozdemir, Zafer O.; Kocaman, Ayse Tuba; Gones, SadikContext: Okra, Abelmoschus esculentus (L.) (Malvaceae), is a medicinal plant widely used in Turkish traditional medicine for the treatment of various diseases such as ulcers and gastritis. Objective: In the present study, we evaluated the gastroprotective effect of okra against ethanol-induced acute gastric mucosal injury in animal models. Materials and methods: Wistar rats were treated with 500, 250 or 100 mg/kg okra; 20 mg/kg famotidine (Fam); and 75 mg/kg quercetin (Que). Following a 60 min period, all the rats were given 1 mL of ethanol (80%). One hour after the administration of ethanol, all groups were sacrificed. Results: At 5000 mg/kg, the extract produced (okra) no signs of toxicity in animals. Okra 500, 250, 100, Fam 20 and Que 75 inhibited ulcer formation by 81.0, 67.5, 67.0, 76.3 and 72.4%, respectively. Okra 500 significantly decreased edema, hemorrhage and inflammation scores compared with the ethanol group (p < 0.05). The oxidant levels decreased significantly in the all study groups compared within ethanol group (p < 0.001). Serum beta-carotene and retinol levels significantly increased 40.2 and 45.4% in the okra 500 group. In okra 500, 250 and Fam 20 groups, apoptosis significantly decreased (p < 0.001), while okra 500, 250 and Fam 20 groups showed a higher percentage of cell proliferation compared with the ethanol group (p < 0.001). Discussion and conclusions: Our in vivo data indicate that okra has a gastroprotective effect against ethanol and could reduce the gastric ulcer as seen from biochemical and histopathological results. We suggest that okra could be a possible therapeutic antiulcer agent.
