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    The Effect of 5-Fu and Ruxolitinib on Mitochondrial Apoptosis in Glioblastoma U87 Cell Line
    (Trakya Üniversitesi, 2020) Aksu, Gonca; Doğanlar, Oğuzhan; Doğanlar, Zeynep Banu
    Aims: The aim of this study is to carry out the effect of 5-Fluorouracil alone or combined with Ruxolitinib on both apoptosis and JAK/STAT pathway in U87 glioblastoma cells. Methods: We used U87 glioblastoma cell lines as the human brain cancer cells. We treated the cells with 5-Fluorouracil (3.125 ?M-400 ?M) alone and with a combination of Ruxolitinib (100 ?M or 400 ?M of Ruxolitinib with 3.125-25 ?M 5-Fluorouracil), and performed the MTT test for calculating IC50 value. Molecular fluorescence staining was performed with Hoechst and acridine orange/ethidium bromide probes. The alteration in mitochondrial apoptosis and JAK/STAT pathways to drug treatment was analyzed by the qRT-PCR assay. Results: Decrease in cell viability was more prominent in U87 cells treated with a combination of 5-Fluorouracil and Ruxolitinib compared to those treated with 5-Fluoro- uracil alone. In gene expression analysis, apoptosis signals were observed in cells treated with 5-Fluorouracil alone and 5-Fluo- rouracil+Ruxolitinib treatment. Conclusion: Treatment with 5-Fluorouracil alone and 5-Fluorouracil+Ruxolitinib combination increased apoptosis in U87 glioblastoma cells. However, it is difficult to mention an evident difference between treatments. Therefore, further studies are needed.
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    Evaluation of Thoracolumbar Injuries in Trakya University School of Medicine According to Aospine Classification System
    (Trakya Üniversitesi, 2020) Aksu, Gonca; Saran, Sercan; Oflaz, Mahmut Sami; Çiftdemir, Mert
    Aims: This study aims to classify the thoracolumbar spinal injuries that were treated in Trakya University School of Medicineaccording to the recent Thoracolumbar AOSpine injury score and crosscheck the classified data with categorical modifiers suchas gender, trauma type and treatment type. Methods: AOSpine Classification System was used to classify thoracolumbar spinalinjuries. Classes were compared with patients’ gender, age, trauma energy and treatment type. Pearson Chi-Squared test and Shapiro-Wilk test were used for statistical analysis. Results: The total number of patients was 248. One hundred fifty-two (61.3%)were male and 96 (38.7%) were female. One hundred and three (86.6%) patients had high-energy trauma and 16 (13.4%) patientshad low-energy trauma in a total of 119 operated patients. Relationship between treatment type and AOSpine Classification System statistically significant. There was also a significant difference between trauma energy and AOSpine Classification Systemtypes. Conclusion: As a conclusion, gender and trauma energy were found to have a relationship and higher energy traumas weremost likely to cause spinal fractures. In addition, AOSpine classification system may be one of the confounding factors regardingthe choice of treatment. Keywords: Classification, spine, injury